quantikine elisa rat il2  (R&D Systems)

Journal: bioRxiv

Article Title: Sustained learned immunosuppression could not prevent local allergic ear swelling in a rat model of contact hypersensitivity

doi: 10.1101/2025.04.05.647348

Figure Lengend Snippet: ( A ) Schematic representation of conditioning paradigm and ( B ) group allocation. Animals underwent three acquisition trials. During these trials the CS, US, and CS 0 groups received saccharin and an injection with CsA (80 mg/kg) prior to sensitization with DNFB. After the incubation time, retrieval started with presentation of the saccharin solution ( CS and Veh group) or water ( US and CS0 group). The US group additionally received 80 mg/kg CsA. On the second day of retrieval, animals were challenged with DNFB and 24 h later ear thickness was measured. ( C ) Taste avoidance index. Conditioned animals showed a pronounced CTA on the second and third acquisition day and over the course of retrieval, reflected by a significantly lower saccharin consumption (ANOVA followed by Bonferroni post hoc analysis; ##p<0.01; ###p<0.001 = Veh vs. all groups; +++p<0.001 = all groups vs. acquisition day 1; ***p<0.001 = CS vs. all groups; n=6-12/group). ( D ) IL-2 levels of ex-vivo anti-CD3 stimulated splenocytes. Compared to the CS0 group, US and CS animals showed reduced IL-2 amounts. Data are presented as percentage of CS0 group (ANOVA followed by Bonferroni post hoc analysis; *p<0.05, ***p<0.001; n=6-12/group). Data are shown as mean ± SEM.

Article Snippet: IL-2 amounts of the supernatants were measured using a sandwich ELISA (Quantikine®ELISA Rat IL2, R&D systems, Minneapolis, USA) according to the manufacturer’s instructions.

Techniques: Injection, Incubation, Ex Vivo

Journal: bioRxiv

Article Title: Sustained learned immunosuppression could not prevent local allergic ear swelling in a rat model of contact hypersensitivity

doi: 10.1101/2025.04.05.647348

Figure Lengend Snippet: ( A ) Schematic representation of conditioning paradigm and ( B ) group allocation. During three acquisition trials (CS/UCS parings), animals of all groups ( CSlow , USlow , US , CS0 ) received saccharin and an injection with CsA (80 mg/kg). Subsequently, animals were sensitized with DNFB. 5 days following sensitization, conditioned animals ( CSlow ) received saccharin paired with injections of 25 % (20 mg/kg) of the full therapeutic CsA dose during retrieval. Control groups received either water together with injections of either 20 mg/kg CsA ( USlow group) or 80 mg/kg CsA ( US group), or vehicle ( CS0 group). Animals were challenged with DNFB on the second day of retrieval. ( C ) Taste avoidance index. Compared to all other groups, conditioned animals ( CSlow ) displayed a pronounced CTA, over the course of retrieval (ANOVA followed by Bonferroni post hoc analysis; +p<0.05, +++p<0.001 = all groups vs. acquisition day 1; ***p<0.001 = CSlow vs. all groups; n=5-6/group). ( D ) US and CSlow groups also significantly differed in IL-2 production of ex-vivo anti-CD3 stimulated splenocytes compared to CS0. Data are presented as percentage of CS0 group. (ANOVA followed by Dunnett’s post hoc analysis, +p<0.05; ++p<0.001; *p<0.05, ***p<0.001; n=5-6/group; Data are shown as mean ± SEM).

Article Snippet: IL-2 amounts of the supernatants were measured using a sandwich ELISA (Quantikine®ELISA Rat IL2, R&D systems, Minneapolis, USA) according to the manufacturer’s instructions.

Techniques: Injection, Control, Ex Vivo

Journal: Biology of reproduction

Article Title: Molecular identification of lyso-glycerophosphocholines as endogenous immunosuppressives in bovine and rat gonadal fluids.

doi: 10.1095/biolreprod.107.064386

Figure Lengend Snippet: FIG. 3. LIP interaction with IL2. A) Com- parison of the effects on spleen T cells of dilutions of ovarian LIP on PHA-stimulated (80 lg/ml) proliferation (-) measured by [3H]-thymidine incorporation and IL2 se- cretion (*-*) measured by ELISA. Values are mean 6 SEM of duplicate wells from a representative experiment performed three times with similar results. Broken horizontal line represents control values obtained in the presence of PHA alone. B) Comparison of the IC50 values for LIP in the three experiments. Values are mean 6 SEM and are not significantly different (P . 0.05). C) Comparison of the effects of dilutions of ovarian LIP on the proliferation of spleen T cells stimulated by 10 lg/ml PHA alone (-) and by 10 lg/ml PHA and 100 pg/ml IL2 (*-

Article Snippet: Interleukin 2 Assay Production of IL2 by rat primary spleen-derived T-cells was assayed using a Quantikine rat IL2 ELISA (R and D Systems), with recombinant rat IL2 as standard and a sensitivity of 15 pg/ml, intra-assay coefficient of variation (CV) of 3.2% and interassay CV of 9.9%.

Techniques: Enzyme-linked Immunosorbent Assay, Control, Comparison